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gdnf  (R&D Systems)


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    R&D Systems gdnf
    Gdnf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+gdnf+duoset+elisa/Human+GDNF+DuoSet+ELISA/pm41163062-129-11-13
    Average 93 stars, based on 49 article reviews
    gdnf - by Bioz Stars, 2026-10
    93/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Cryogel microcarriers for sustained local delivery of growth factors to the brain.
    Article Snippet: .. ELISA kits were purchased from R&D Systems, Human GDNF DuoSet ELISA #DY212, Human BDNF DuoSet ELISA # DY248 and DuoSet ELISA Ancillary Reagent Kit 2 #DY008. ..

    Article Title: Intravenously Infusing the Secretome of Adipose-Derived Mesenchymal Stem Cells Ameliorates Neuroinflammation and Neurological Functioning After Traumatic Brain Injury.
    Article Snippet: .. Concentrations of vascular endothelial growth factor (VEGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), plateletderived growth factor (PDGF)-BB, and intercellular cell adhesion molecule (ICAM)-1 in the ASC-ST were measured using a Human VEGF Quantikine ELISA Kit (Catalog #: SVE00, R&D Systems, USA), Human Free BDNF Quantikine ELISA Kit (Catalog #:SBD00, R&D Systems, USA), Human GDNF DuoSet ELISA (Catalog #:DY212, R&D Systems, USA), Human PDGF-BB Quantikine ELISA Kit (Catalog #: SBB00, R&D Systems, USA), and Human ICAM-1/CD54 DuoSet ELISA Kit (Catalog #: DY720, R&D Systems, USA) following the manufacturers’ instructions. .. Optical Density (OD) was measured with MultiskanTM FC (Thermo Scientific, D ow nl oa de d by B IU M U ni v Pa ri s 5 R en e D es ca rt es B iu m f ro m w w w .li eb er tp ub .c om a t 1 2/ 15 /1 9.

    Article Title: Unmasking a new prognostic marker and therapeutic target from the GDNF-RET/PIT1/p14ARF/p53 pathway in acromegaly
    Article Snippet: .. Secreted GDNF levels were measured with a Human GDNF DuoSet ELISA (DY212, R&D Systems, USA) according to the manufacturer's protocol. ..



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    Fig. 5. Protein loading into cryogel microcarriers is dependent on SPA content. 100 ng of <t>GDNF</t> (A) or BDNF (B) were added to the cryogel microcarriers. <t>ELISA</t> analysis of the supernatants the following day showed no GDNF loading to 60% SPA cryogels while 95% cryogels showed the highest loading, whereas all types of cryogels were able to load BDNF. n = 3 for all experiments and error bars represent ± standard deviation *P < 0.05 and ****P < 0.0001 via one-way ANOVA.
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    Fig. 5. Protein loading into cryogel microcarriers is dependent on SPA content. 100 ng <t>of</t> <t>GDNF</t> (A) or BDNF (B) were added to the cryogel microcarriers. <t>ELISA</t> analysis of the supernatants the following day showed no GDNF loading to 60% SPA cryogels while 95% cryogels showed the highest loading, whereas all types of cryogels were able to load BDNF. n = 3 for all experiments and error bars represent ± standard deviation *P < 0.05 and ****P < 0.0001 via one-way ANOVA.
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    Fig. 5. Protein loading into cryogel microcarriers is dependent on SPA content. 100 ng <t>of</t> <t>GDNF</t> (A) or BDNF (B) were added to the cryogel microcarriers. <t>ELISA</t> analysis of the supernatants the following day showed no GDNF loading to 60% SPA cryogels while 95% cryogels showed the highest loading, whereas all types of cryogels were able to load BDNF. n = 3 for all experiments and error bars represent ± standard deviation *P < 0.05 and ****P < 0.0001 via one-way ANOVA.
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    Fig. 5. Protein loading into cryogel microcarriers is dependent on SPA content. 100 ng of GDNF (A) or BDNF (B) were added to the cryogel microcarriers. ELISA analysis of the supernatants the following day showed no GDNF loading to 60% SPA cryogels while 95% cryogels showed the highest loading, whereas all types of cryogels were able to load BDNF. n = 3 for all experiments and error bars represent ± standard deviation *P < 0.05 and ****P < 0.0001 via one-way ANOVA.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Cryogel microcarriers for sustained local delivery of growth factors to the brain.

    doi: 10.1016/j.jconrel.2024.03.023

    Figure Lengend Snippet: Fig. 5. Protein loading into cryogel microcarriers is dependent on SPA content. 100 ng of GDNF (A) or BDNF (B) were added to the cryogel microcarriers. ELISA analysis of the supernatants the following day showed no GDNF loading to 60% SPA cryogels while 95% cryogels showed the highest loading, whereas all types of cryogels were able to load BDNF. n = 3 for all experiments and error bars represent ± standard deviation *P < 0.05 and ****P < 0.0001 via one-way ANOVA.

    Article Snippet: ELISA kits were purchased from R&D Systems, Human GDNF DuoSet ELISA #DY212, Human BDNF DuoSet ELISA # DY248 and DuoSet ELISA Ancillary Reagent Kit 2 #DY008.

    Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

    Fig. 7. 80% SPA cryogel microcarriers can release GDNF over 4 months and can be refilled. Different amounts of GDNF (100, 500, 1000, 2000 ng) were added to 0.01 mg cryogels (amount loaded in inset graph) and cumulative GDNF release (ng) was analyzed over 9 months. When all the GDNF had been released at week 18 the 80% SPA cryogel microcarriers were washed 4× with PBS and reloaded (right hand inset graph) with different amounts of GDNF (100, 500, 1000, 2000 ng) showing a similar loading amount and release profile as in the first time. n = 3 for all experiments and error bars represent ± standard deviation.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Cryogel microcarriers for sustained local delivery of growth factors to the brain.

    doi: 10.1016/j.jconrel.2024.03.023

    Figure Lengend Snippet: Fig. 7. 80% SPA cryogel microcarriers can release GDNF over 4 months and can be refilled. Different amounts of GDNF (100, 500, 1000, 2000 ng) were added to 0.01 mg cryogels (amount loaded in inset graph) and cumulative GDNF release (ng) was analyzed over 9 months. When all the GDNF had been released at week 18 the 80% SPA cryogel microcarriers were washed 4× with PBS and reloaded (right hand inset graph) with different amounts of GDNF (100, 500, 1000, 2000 ng) showing a similar loading amount and release profile as in the first time. n = 3 for all experiments and error bars represent ± standard deviation.

    Article Snippet: ELISA kits were purchased from R&D Systems, Human GDNF DuoSet ELISA #DY212, Human BDNF DuoSet ELISA # DY248 and DuoSet ELISA Ancillary Reagent Kit 2 #DY008.

    Techniques: Standard Deviation

    Fig. 6. GDNF loaded to 95% SPA cryogel microcarriers is released slowly for seven months. Different amounts of GDNF (100, 500, 1000, 2000 ng) were added to 0.01 mg cryogels and the total amount loaded for each amount is shown in the inset figure. Cumulative GDNF release (ng) over 7 months in PBS + 0.1% BSA was measured at each time point via ELISA. For 2000 ng GDNF, around 46% of loaded GDNF was released by week 28 (longest time tested), n = 3 for all experiments and error bars represent ± standard deviation.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Cryogel microcarriers for sustained local delivery of growth factors to the brain.

    doi: 10.1016/j.jconrel.2024.03.023

    Figure Lengend Snippet: Fig. 6. GDNF loaded to 95% SPA cryogel microcarriers is released slowly for seven months. Different amounts of GDNF (100, 500, 1000, 2000 ng) were added to 0.01 mg cryogels and the total amount loaded for each amount is shown in the inset figure. Cumulative GDNF release (ng) over 7 months in PBS + 0.1% BSA was measured at each time point via ELISA. For 2000 ng GDNF, around 46% of loaded GDNF was released by week 28 (longest time tested), n = 3 for all experiments and error bars represent ± standard deviation.

    Article Snippet: ELISA kits were purchased from R&D Systems, Human GDNF DuoSet ELISA #DY212, Human BDNF DuoSet ELISA # DY248 and DuoSet ELISA Ancillary Reagent Kit 2 #DY008.

    Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

    Fig. 9. Cytocompatibility of PEGDA-SPA cryogels with human neuronal cells, human microglial cells, and rat primary neural (E14 VM) cultures. SH-SY5Y cells, HMC3 cells and E14 VM cultures were incubated with 80% SPA cryogels empty (CTL) or loaded with 500 ng GDNF (Ai, Bi, Ci) or 20% SPA cryogels unloaded or loaded with 500 ng BDNF (Aii, Bii, Cii). Viability analyses confirmed the cytocompatibility of the cryogels and demonstrated a pronounced trophic effect on the primary neural cultures. Visualization of the VM cultures at Day 5 (using immunofluorescence for beta-III tubulin) (D) confirmed a healthy neuronal population in the presence of the cryogels. Scale bar represents 150 μm. Data are represented as mean ± SEM of n = 3 biological replicates. ****P < 0.0001, ***P < 0.001 by two-way repeated measures ANOVA.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Cryogel microcarriers for sustained local delivery of growth factors to the brain.

    doi: 10.1016/j.jconrel.2024.03.023

    Figure Lengend Snippet: Fig. 9. Cytocompatibility of PEGDA-SPA cryogels with human neuronal cells, human microglial cells, and rat primary neural (E14 VM) cultures. SH-SY5Y cells, HMC3 cells and E14 VM cultures were incubated with 80% SPA cryogels empty (CTL) or loaded with 500 ng GDNF (Ai, Bi, Ci) or 20% SPA cryogels unloaded or loaded with 500 ng BDNF (Aii, Bii, Cii). Viability analyses confirmed the cytocompatibility of the cryogels and demonstrated a pronounced trophic effect on the primary neural cultures. Visualization of the VM cultures at Day 5 (using immunofluorescence for beta-III tubulin) (D) confirmed a healthy neuronal population in the presence of the cryogels. Scale bar represents 150 μm. Data are represented as mean ± SEM of n = 3 biological replicates. ****P < 0.0001, ***P < 0.001 by two-way repeated measures ANOVA.

    Article Snippet: ELISA kits were purchased from R&D Systems, Human GDNF DuoSet ELISA #DY212, Human BDNF DuoSet ELISA # DY248 and DuoSet ELISA Ancillary Reagent Kit 2 #DY008.

    Techniques: Incubation, Immunofluorescence

    Fig. 12. PEGDA-SPA cryogels deliver GDNF to the brain. GDNF delivery, retention and release from implanted cryogels was assessed by measuring the optical density of GDNF immunostaining at the implant site (A, D) and in the peri-implant region (B, D), as well as the volume of GDNF staining (C, D) at Days 7 and 14 post- implantation. In comparison with bolus administration, GDNF immunostaining was clearly visible both within, and surrounding, the implant site in the brains injected with GDNF-loaded cryogels. Scale bar represents 1 mm and 200 μm (inset). Data are represented as mean ± SEM of n = 6 rats. ****P < 0.0001, ***P < 0.001, *P < 0.05 by two-way ANOVA with Tukey post-hoc test.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Cryogel microcarriers for sustained local delivery of growth factors to the brain.

    doi: 10.1016/j.jconrel.2024.03.023

    Figure Lengend Snippet: Fig. 12. PEGDA-SPA cryogels deliver GDNF to the brain. GDNF delivery, retention and release from implanted cryogels was assessed by measuring the optical density of GDNF immunostaining at the implant site (A, D) and in the peri-implant region (B, D), as well as the volume of GDNF staining (C, D) at Days 7 and 14 post- implantation. In comparison with bolus administration, GDNF immunostaining was clearly visible both within, and surrounding, the implant site in the brains injected with GDNF-loaded cryogels. Scale bar represents 1 mm and 200 μm (inset). Data are represented as mean ± SEM of n = 6 rats. ****P < 0.0001, ***P < 0.001, *P < 0.05 by two-way ANOVA with Tukey post-hoc test.

    Article Snippet: ELISA kits were purchased from R&D Systems, Human GDNF DuoSet ELISA #DY212, Human BDNF DuoSet ELISA # DY248 and DuoSet ELISA Ancillary Reagent Kit 2 #DY008.

    Techniques: Immunostaining, Staining, Comparison, Injection

    Fig. 14. Morphology of PEGDA-SPA cryogels in situ in the brain. The spherical structure of the cryogels was retained after transplantation into the rat striatum. Their size was consistent overall, a small increase in measured diameter was seen in the GDNF-loaded cryogels (A, B) while no change in diameter size in BDNF-loaded cryogels (C, D) at Day 14 after transplantion. Scale bar is 50 μm. Data are represented as mean ± SEM of n = 6 rats (5 cryogels measured per rat). *P < 0.05 by two- way ANOVA.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Cryogel microcarriers for sustained local delivery of growth factors to the brain.

    doi: 10.1016/j.jconrel.2024.03.023

    Figure Lengend Snippet: Fig. 14. Morphology of PEGDA-SPA cryogels in situ in the brain. The spherical structure of the cryogels was retained after transplantation into the rat striatum. Their size was consistent overall, a small increase in measured diameter was seen in the GDNF-loaded cryogels (A, B) while no change in diameter size in BDNF-loaded cryogels (C, D) at Day 14 after transplantion. Scale bar is 50 μm. Data are represented as mean ± SEM of n = 6 rats (5 cryogels measured per rat). *P < 0.05 by two- way ANOVA.

    Article Snippet: ELISA kits were purchased from R&D Systems, Human GDNF DuoSet ELISA #DY212, Human BDNF DuoSet ELISA # DY248 and DuoSet ELISA Ancillary Reagent Kit 2 #DY008.

    Techniques: In Situ, Transplantation Assay

    Fig. 5. Protein loading into cryogel microcarriers is dependent on SPA content. 100 ng of GDNF (A) or BDNF (B) were added to the cryogel microcarriers. ELISA analysis of the supernatants the following day showed no GDNF loading to 60% SPA cryogels while 95% cryogels showed the highest loading, whereas all types of cryogels were able to load BDNF. n = 3 for all experiments and error bars represent ± standard deviation *P < 0.05 and ****P < 0.0001 via one-way ANOVA.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Cryogel microcarriers for sustained local delivery of growth factors to the brain.

    doi: 10.1016/j.jconrel.2024.03.023

    Figure Lengend Snippet: Fig. 5. Protein loading into cryogel microcarriers is dependent on SPA content. 100 ng of GDNF (A) or BDNF (B) were added to the cryogel microcarriers. ELISA analysis of the supernatants the following day showed no GDNF loading to 60% SPA cryogels while 95% cryogels showed the highest loading, whereas all types of cryogels were able to load BDNF. n = 3 for all experiments and error bars represent ± standard deviation *P < 0.05 and ****P < 0.0001 via one-way ANOVA.

    Article Snippet: ELISA kits were purchased from R&D Systems, Human GDNF DuoSet ELISA #DY212, Human BDNF DuoSet ELISA # DY248 and DuoSet ELISA Ancillary Reagent Kit 2 #DY008.

    Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

    Fig. 6. GDNF loaded to 95% SPA cryogel microcarriers is released slowly for seven months. Different amounts of GDNF (100, 500, 1000, 2000 ng) were added to 0.01 mg cryogels and the total amount loaded for each amount is shown in the inset figure. Cumulative GDNF release (ng) over 7 months in PBS + 0.1% BSA was measured at each time point via ELISA. For 2000 ng GDNF, around 46% of loaded GDNF was released by week 28 (longest time tested), n = 3 for all experiments and error bars represent ± standard deviation.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Cryogel microcarriers for sustained local delivery of growth factors to the brain.

    doi: 10.1016/j.jconrel.2024.03.023

    Figure Lengend Snippet: Fig. 6. GDNF loaded to 95% SPA cryogel microcarriers is released slowly for seven months. Different amounts of GDNF (100, 500, 1000, 2000 ng) were added to 0.01 mg cryogels and the total amount loaded for each amount is shown in the inset figure. Cumulative GDNF release (ng) over 7 months in PBS + 0.1% BSA was measured at each time point via ELISA. For 2000 ng GDNF, around 46% of loaded GDNF was released by week 28 (longest time tested), n = 3 for all experiments and error bars represent ± standard deviation.

    Article Snippet: ELISA kits were purchased from R&D Systems, Human GDNF DuoSet ELISA #DY212, Human BDNF DuoSet ELISA # DY248 and DuoSet ELISA Ancillary Reagent Kit 2 #DY008.

    Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation